Research Training Program

Lawrence Lopez
Universidad Nacional - Agraria La Molina
Peru

Michael Braun, Ph.D.
Supervising Scientist
Department of Vertebrate Zoology
Division of Birds

"An excellent program. It really prepares you for doing "hot" research."

The Use of Genetic Markers to Characterize Hybridization Between Black-Capped and Carolina Chickadees

This research focused on investigating hybridization between Black-capped and Carolina chickadees. The Restriction Fragment Length Polymorphism (RFLP) method was used to analyse DNA obtained from 100 bird specimens. Laboratory work included extraction, digestion with restriction enzymes,and electrophoresis of DNA, southern blotting and probing of blots. MtDNA, C7 and Ski were the markers used. To analyse the results, the genotype of each individual was calculated and a composite genotype for each individual was obtained. Next, the percentage of hybrids and the frequency of Black-capped and Carolina alleles in each population was calculated. With these results, it was possible to determine the amount of genetic introgression that exists in this particular hybrid zone. With comparisons done with other hybrid zones, it was possible to establish the dynamic and maintenance of the contact zone between Black-capped and Carolina chickadees at different locations.

This research was supported by a grant from the National Science Foundation Research Experiences for Undergraduates Program ( Award: DIR-9200203).